The bank announced a figure of $4.98 billion for the first half of 2009, an eight per cent increase on the amount recorded in the same period of 2008.
Income rose 37 per cent to $27.2 billion, with Barclays' investment management and banking division recording a 44 per cent increase in pre-tax profit.
However, performance of its retail banking division was weaker, with this area of the business seeing profits fall by more than half to $448 million.
Chief executive John Varley said that Barclays has benefited from a "diversified business base" in what have been "challenging market conditions".
"Our goal for 2009 is very clear: we seek to deliver another year of solid profitability. Our first half performance is a good start to this," he remarked.
Last week, the bank's website was hit by a glitch that prevented some customers from accessing their accounts online.