The new Blackstone Capital Partners V fund exceeds that raised by the private equity division of Goldman Sachs, which raised $20 billion and was the previous record holder.
The fund is to be invested across a number of markets and industry sectors, with two-thirds of its available capital has already been earmarked for existing deals.
These are thought to include the buyout company's recent purchase of Hilton Hotels and Freescale Semiconductors for $26 billion and $18 billion respectively.
Blackstone chief executive office Stephen Schwarzman said: "This further vote of confidence from our investors is extremely gratifying.
"The record-breaking amount of capital at our disposal allows us to continue our leadership role in private equity investing on a global basis."
The influx of capital brings Blackstone's total funds raised to around $67 billion since it launched in 1987.