Contactless payment terminals will be rolled out to the stores during the second half of 2009, computing.co.uk reports.
The technology will allow customers to pay for purchases under Â£10 ($15.25) by holding up a contactless-enabled payment card to a reader without having to enter a pin number.
According to the site, the upcoming 2012 Olympic Games is acting as a catalyst for the adoption of contactless systems as providers look for ways to speed up payments for items such as cab fares, coffees and food.
The adoption of contactless payments by large retailers like Boots is seen as crucial to the development of the technology, it added.
Barclaycard welcomed the announcement of the trial. The company's managing director of UK cards, Amer Sajed, said: "Consumer feedback highlights the increasing demand for contactless payments and we hope other retailers will follow Boots and introduce contactless payment facilities in store in our continuing aim to achieve a contactless Britain."
According to Barclaycard, 98 per cent of contactless cardholders say the technology is easy to use.