The loan is at a rate of 6.75 per cent, which is one per cent higher than the normal borrowing level.
Barclays blamed a technical hitch in London's settlement for the loan rather than a problem a problem with liquidity.
The bank said it was "flush with liquidity".
Barclays has to settle their trading through the market settlement system, this means that if borrowings are greater than reserves then banks can borrow money from others to settle the balance.
A malfunction meant that Barclays had a shortfall too late to be able to borrow from another bank meaning that they had to ask the Bank of England.
Barclays said: "In these challenging times the dramatisation of such situations is of no help to markets, their members or their customers."
There are 45 banks eligible to go to the Bank of England for this type of loan, and high street banks have called upon the central bank 14 times this year for help.